ABSTRACT

INTRODUCTION:

We aimed to determine if naloxone has any additive effect in the treatment of amitraz poisoning and clinical and laboratory findings of the toxicity in our study.

METHODS:

In the experiment 60 adult female Wistar Albino rats of which mean weight 220- 260 gr were used. The rats were divided to four groups. Eight hundred mg/kg amitraz was administered to groups 1. 3. and 4. by orogastric route while group 2 was control. The change in respiratory and heart rates of four groups were recorded every twenty minutes. When toxicity symptoms in groups 3 and 4 were observed, the treatment was initiated. As treatment 20 ml/kg normal saline and 0,1 mg/kg atropine was administered when heart rate decreased under 250 beats/minutes intraperitonealy. 0,3 mg/kg naloxone was given additionally to group 4. All of the rats were sacrified under general anesthesia by obtaining intracardiac blood samples for hematological and biochemical analyses. Complete blood count (CBC) which contains white blood cell (Wbc), red blood cell (Rbc), hemoglobin (Hgb), hematocrit (Hct) and platelet (Plt) values as biochemical analyses blood urea nitrogen (BUN), creatine (crea), sodyum (Na+), calcium (Ca+), glucose (Glu), serum glutamic oxalasetic transaminase (SGOT), serum glutamic piruvic transaminase (SGPT) values were asseyed. The data were evaluated by SPSS 13.0.

RESULTS:

In hematological evaluation there was statisticaly significant difference between groups for the values of Wbc, Rbc, Hct, Hgb as well as in the biochemical evaluation for BUN, crea, Glu, Na, SGOT, SGPT (p<0,05). Naloxone treatment was not superior to supportive treatment statisticaly.

DISCUSSION AND CONCLUSION:

In conclusion hematological and biochemical values varies in amitraz poisoning and this can be cured by supportive treatment. Naloxone has no additive effect to the treatment.